Pathogenes Inc.

Pathogenes Inc.
PO Box 970, Fairfield, Fl. 32634
15471 NW 112th Ave, Reddick, Fl. 32686

ph: 352-591-3221
fax: 352-591-4318
sellison@pathogenes.com

Technical Data

Our novel technologies are available for license. To discuss the technology, licenses, or IP please download the CA and fax or email the signed form to 352-591-4318 or www.pathogenes.com.

Peptide Based Serological Testing
October 1
New technology may differentiate symptomatic from asymptomatic S. neurona infections. The use of strain identification may lead to discerning highly neurovirulent, neuroinvasive S. neurona from low virulent infections in the horse. We have identified two unique proteins that discern all phenotypes of S. neurona that infect horses. These proteins do not bind antibodies to species other than neurona in the genus sarcocystis. The technology has been licensed and the test is available for purchase. The individual peptides or proteins can be purchased for research use. Contact us for details.
Evidence that horses have antibodies to S. falcatula and S. neurona
The Missouri Horse
Horses either have antibodies to S. falcatula from infections with this organism or antibodies raised to Sarcocystis infections (S. fayeri) cross-react with some antigens of S. neurona, probably SnSAG4. The author (Marsh AE, Vet Parasit 95 (2001) 143-154 stated that S. falcatula antigens can be recognized by S. neurona infected horses, the oposite may be indicated here.
Sequence Data
S. neurona SAG1
S. neurona SAG5
S. neurona SAG6
Patents
US 6780415
US 6808714
US 7250286 and divisionals, world wide patents
SnSAG1
Protein data
29 kDa, pI 7.48
Sensitivity & Specificity
SnSAG5
Protein data
53.3, pI 6.6
protein mw 26.2 kDa
SfSAG6
Protein data
54.4, pI 6.9
protein mw 27.3

sequence data- NA and protein
Gene Blast Data
Sarcocystis neurona gene data
Nucleotide & amino acid sequence comparison
Equine Biomarkers
Sarcocystis neurona antibody test kit ELISA PDF files.
PDF format instructions for the following kits may be downloaded. Enter your license number to allow access for Sarcocystis neurona antibody test kit ELISA
___-______-______
No license is required for the Sarcocystis neurona antibody test kit ELISA SAG 1, 5, 6. License fees are included in the cost of the plate.
Sensitivity and specificity of SAG 1 ELISA
9-10-2010
The sensitivity and specificity of the SAG 1 ELISA was determined in induced infections using SnSAG 1 S. neurona merozoites. Twenty-four and 14 chronic experimentally induced EPM cases were evaluated. The parameters included AI SAG 1 index, SAG 1 ELISA, lameness determined by blinded exam, ataxia determined by blinded examination, EPM score, and EBI immunoblot. See the tabulated data

Missed the Questions/Answers? Find them here.

Available technology limits effective treatment and prevention of EPM in horses. However, novel solutions are available through a veterinary client patient relationship (VCP) under code of Federal Regulation (9 CFR 107.1).

Oroquin-10

Oroquin-10 is a highly effective anti-coccidiocidal drug compounded with an immune stimulant, levamisole. The quinalone drug kills intracellular protozoa more rapidly and at a lower dose than conventional agents used to treat EPM by targeting protozoan mitochondria. In vitro cell assays determined that S. neurona and Neospora hughesi are eliminated at one tenth the dose of other coccidial agents used in EPM. The oral compound begins killing within 1 day. A prevention stratagy can be used to eliminate chronic relapsing EPM at 1/100th the costs of conventional therapy. Click here to see more...

Pathogenes Inc.
PO Box 970, Fairfield, Fl. 32634
15471 NW 112th Ave, Reddick, Fl. 32686

ph: 352-591-3221
fax: 352-591-4318
sellison@pathogenes.com

Pathogenes Inc.

Technical Data

Peptide Based Serological Testing

Evidence that horses have antibodies to S. falcatula and S. neurona

Sequence Data

Patents

SnSAG1

SnSAG5

SfSAG6

Gene Blast Data

Sarcocystis neurona antibody test kit ELISA PDF files.

Sensitivity and specificity of SAG 1 ELISA